Figure 1

Immunoblot patterns produced by anti-α-enolases and anti-tRNP^(Ser)Sec antibodies on electrophoretically separated primate and rat liver homogenates and dot blot results with recombinant tRNP^(Ser)Sec. In both rat and primate liver preparations, a band of ~48 kDa is immunofixed by a polyclonal goat IgG anti-α-enolase specific antibody; a band of ~50 kDa is immunofixed by a serum containing a high-titre anti-tRNP^(Ser)Sec antibody. Anti-α-enolase antibody does not recognize tRNP^(Ser)Sec by dot blot analysis.