Immunofluorescent detection of IFN-γ, TNF-α, IL-1β, iNOS and nitrotyrosine in the CNS of female Lewis rats. The spinal cords were isolated when the animals were showing maximum clinical symptoms (i.e. for the EAE group clinical score of 5 and EAE+NAC clinical score of 3). Immunostaining was performed in spinal cord sections (n = 12) of female Lewis rats as described in Materials and Methods. EAE sections showed intense staining for IFN-γ, TNF-α, iNOS and nitrotyrosine with less intense staining for IL-1 β. Control and EAE + NAC sections showed minimal staining (original magnification 200×).